Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
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چکیده
منابع مشابه
Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
The pioneering work by Patrick H. O'Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry1975, 250, 4007-4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the prote...
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Two-dimensional electrophoresis (2-DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sort’s protein according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the se...
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Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist. The events that have led to the birth of proteomics ...
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Featured article: O’Farrell PH. High resolution twodimensional electrophoresis of proteins. J Biol Chem 1975;250:4007–21. No one had heard of proteomics in 1971. The invention of the word was 25 years away (1 ). At the time, doing molecular biology meant studying bacteria or their viruses, the bacteriophage. But as a starting graduate student in Boulder, Colorado, I wanted to study eukaryotic e...
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ژورنال
عنوان ژورنال: Proteomes
سال: 2016
ISSN: 2227-7382
DOI: 10.3390/proteomes4030027